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KMID : 0616120070380010015
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Korean Journal of Pharmacognosy
2007 Volume.38 No. 1 p.15 ~ p.18
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Quantitative Analysis Comparison of Korea and China Scrophulariae Radix
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Lee Hye-Won
Park So-young
Choo Byung-kil
Chun Jin-Mi
Lee A-Yeong
Kim Ho-kyoung
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Abstract
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Scrophulariae Radix(Scrophulariaceae) is a perennial growing in all parts of korea, China and Asia. It has been used as a folk medicine in treating guttural diseases acting as an antiphlogistics, antipyretics and analgesics. In this report, to compare with Korea and China product, quantitative analysis of harpagoside and p-methoxy cinnamic acid(4-MCA) was performed by HPLC method. Using a Luna C_{18}(2) column, we carried out quantitative analysis with 0.1% phosphoric acid in water and acetonitrile as gradient condition. Harpagoside and 4-MCA were detected at retention time of about 11.5 and 15.0 min, respectively. Contents of harpagoside and 4-MCA proved that Korean products were higher than China more than twice.
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KEYWORD
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Scrophulariae Radix, Scrophulariaceae, harpagoside, p-methoxycinnamic acid(4-MCA), HPLC
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